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兔眼蓝莓快繁技术研究

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以兔眼蓝莓品种‘园蓝’、‘杰兔’、‘灿烂’茎段为材料,进行组织培养试验,成功建立起高效的组织培养技术体系。结果表明:不同植物生长调节剂对兔眼蓝莓不定芽的增殖影响不同,其中ZT在不定芽增殖中是主导因子, ZT浓度2.0 mg/L,最适宜兔眼蓝莓不定芽的诱导增殖;NAA对苗高有明显的影响, NAA浓度高于0.005 mg/L时,随着NAA浓度的升高,苗高会出现显著的降低;品种差异对不定芽诱导没有明显的影响。兔眼蓝莓不定芽增殖的最优条件为WP M+2.0 mg/L ZT+0.010 mg/L NAA,增殖系数高,达到了4.12,不定芽长势较好。最佳生根培养基为1/2WPM+2.0 mg/L IBA,生根率达到了92.10%,生根数多,根的长势良好。
Rapid Propagation of Vaccinium ashei
The shoots of ‘Gardenblue’ , ‘Premier’ and ‘Brightwell’ of blueberry ( Vaccinium ashei) were chosen as experimental materials and tissue culture system was set up successfully in the study. The results of the study were as follows:Different hormone had different effects on adventitious buds in multiplication. The effects of ZT dominated in all factors associated, and 2. 0 mg/L ZT was the most apt for shoot proliferation. NAA had a signif-icant effect on the shoot height. When NAA concentrations were higher than 0. 005 mg/L, with the NAA concentra-tion increased, the shoot height would be reduced significantly. Difference between varieties had no obvious effect on adventitious bud induction. The shoots cultured in the medium supplemented with 2. 0 mg/L ZT and 0. 010 mg/L NAA had the best performance. Proliferation rate reached 4. 12, and shoots grew well. The shoots cultured in the 1/2 WPM medium supplemented with 2. 0 mg/L IBA were appropriate, and it achieved the best rooting rate of the 92. 10%, and the roots kept good condition of growth.

Vaccinium asheivarietytissue culturerapid propagation

朱芳明、张太奎、刘惠民、刘小珍、张汉尧

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西南林业大学西南地区生物多样性保育国家林业局重点实验室,云南 昆明650224

西南林业大学云南省高校林木遗传改良与繁育重点实验室,云南 昆明650224

兔眼蓝莓 品种 组织培养 快繁体系

西南林业大学云南省省级重点学科(林学)西南地区生物多样性保育国家林业局重点实验室开放基金

2016

西南林业大学学报
西南林业大学

西南林业大学学报

CSTPCD北大核心
影响因子:0.773
ISSN:2095-1914
年,卷(期):2016.36(3)
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