In vitro Regeneration System of ‘Hort16A’ Kiwifruit Introduced from Abroad
The tissue culture regeneration system of kiwifruit ‘Hort16A’ ( Actinidia chinensis ‘Hort16A’ ) in-troduced from abroad was established. The callus formation rate and adventitious bud differentiation rate were investi-gated using the L9(34) orthogonal experiment, proliferation of adventitious buds were further studied using single fac-tor randomized block design of experiment, and the rooting of kiwifruit was researched using the L9(34) orthogonal experiment. The result indicated that the highest callus formation rate (97. 78%± 1. 92%) was investigated from the explants in the MS medium contained 0. 05 mg/L NAA and 0. 3 mg/L ZT after culturing 20 d. That the differentiation rate and height of adventitious buds were (84. 44%± 1. 92%) and (1. 52 ± 0. 29) cm respectively were the best dif-ferentiation effect surveyed on the explants in this medium after another 14 d culturing. The biggest proliferation rate (3. 67 ± 0. 33) and height (2. 93 ± 0. 12) cm of adventitious buds were surveyed on the buds in the MS medium con-tained 1. 5 mg/L ZT after culturing 30 d. The best rooting that rooting ratio, root length, root width and number of roots generated per shoot were (98. 9%± 1. 9%), (7. 60 ± 0. 44) cm, (0. 47 ± 0. 07) cm and (7. 67 ± 2. 19) respec-tively was investigated from the shoots in the 1/2 MS medium contained 0. 7 mg/L IBA and 0. 1 g/L activated carbon.
kiwifruittissue culturecallusdifferentiation of adventitious budsrooting culture