Analysis of Paulownia NLR Gene Family and their Responses to Phytoplasmas
In order to study the role of PfNLR gene family in Paulownia fortunei resistance to Paulownia witches'broom(PaWB),bioinformatics methods were used to comprehensively analyze the composition,physico-chemical properties,chromosomal localization,evolution,structure,cis-acting elements and tissue expression spe-cificity of P.fortunei NLR(PfNLR)family members.Combined with the miRNA and transcriptome data before and after phytoplasma infecting Paulownia,the genes associated with resistance to PaWB were screened.The res-ults showed that 199 PfNLRs in P.fortunei belonged to 6 subfamilies;91.9％of PfNLRs were located on 18 chro-mosomes of P.fortunei,of which 62.8％were clustered,among them,there were 10 chromosome segment re-peats and 1 chromosome tandem repeats in 23 PfNLRs;PfNLR contains hormonal and stress elements;integrated transcriptome and miRNA analysis found that there were 156 pairs of targeted relationships between PfNLRs and miRNAs;most of PfNLRs were expressed in buds and roots;of which,16 PfNLRs were significantly different be-fore and after phytoplasma infection.Further analysis revealed that PfNLR181 mediated by pf-miRNA482 played a role in resistance of PaWB.These results could provide a theoretical basis for analyzing the function of PfNLR protein.
万方数据
维普
