羧甲基壳聚糖诱导葡萄柚果实抗病防御代谢物差异分析
Analysis of Resistance and Defense Metabolites of Postharvest Citrus paradisi Induced by Carboxymethyl-chitosan
刘胜红 1杨清 1吴化雨 1唐勇 1王芳 2李根前 1邓佳3
作者信息
- 1. 西南林业大学林学院,云南昆明 650233
- 2. 西南林业大学林学院,云南昆明 650233;西南地区生物多样性保育国家林业和草原局重点实验室,云南昆明 650233
- 3. 西南林业大学林学院,云南昆明 650233;西南山地森林资源保育与利用教育部重点实验室,云南昆明 650233
- 折叠
摘要
为研究羧甲基壳聚糖(CMCS)诱导采后葡萄柚果实抗性代谢物的差异,以葡萄柚果实为试材,采用1.5％CMCS和无菌水(CK)分别诱导葡萄柚24 h,利用非靶向代谢组学技术分析葡萄柚果实中代谢物的差异.结果表明,CMCS诱导葡萄柚24h,共得到92种差异代谢物,其中,上调36种,下调56种.KEGG通路注释结果表明,差异代谢物被显著(P＜0.05)富集到21条通路中,主要参与氨基酸和苯丙烷代谢.进一步筛选发现L-谷氨酰胺、L-谷氨酸、L-天冬酰胺、L-精氨酸、酪胺、丙二酸、莽草酸、D-赤藓-3-甲基苹果酸、反式肉桂酸和香豆素的表达量发生显著变化,且显著(P＜0.05)高于CK.另将相关差异代谢物与关键抗性差异基因(HSP17.4B、NCED1、GGCT2;2、HSP22.0、WRKY53、HSP22.0、WRKY40、WRKY54)进行相关性分析,结果证实两者之间存在显著正相关关系.
Abstract
In order to study the difference of Carboxymethyl-chitosan(CMCS)-induced resistant metabolites inpostharvestgrapefruitfruits.Inthisstudy,grapefruitfruitswereusedastestmaterials,andgrapefruitwasinducedbyl.5％CMCS and sterile water(CK)for 24 h,respectively.The differences of metabolites in grapefruit fruits were ana-lyzed by non-targeted metabolomics.The results showed that a total of 92 differential metabolites were obtained after CMCS induced grapefruit for 24 h,of which 36 were up-regulated and 56 were down-regulated.The KEGG pathway annotation results showed that the differential metabolites were significantly(P＜0.05)enriched into 21 pathways,mainly involved in amino acid and phenylpropanoid metabolism.Further screening found that the ex-pression levels of L-Glutamine,L-Glutamic acid,L-Asparagine,Arginine,Tyramine,Malonic acid,Shikimic acid,D-erythro-3-Methylmalate,trans-Cinnamate and Coumarin changed significantly,and were significantly(P＜0.05)higher than CK.In addition,the related differential metabolites and key resistance differential genes(HSP17.4B,NCED1,GGCT2;2 HSP22.0,WRKY53,HSP22.0,WRKY40,WRKY54)confirmed that there was a significant positive correlation between the two.
关键词
羧甲基壳聚糖/代谢组/采后防治/氨基酸代谢/苯丙烷代谢Key words
Carboxymethyl-chitosan/metabolome/prevention and control after mining/amino acid metabol-ism/phenylpropane metabolism引用本文复制引用
基金项目
国家自然科学基金(31960326)
国家自然科学基金(32160394)
云南省高层次人才培养支持计划青年拔尖人才专项(YNWR-QNBJ-2020-205)
云南省"两类"人才培养项目(202105AC160045)
云南省农业联合专项(202101BD070001-065)
出版年
2024
国家科技期刊平台
NSTL
万方数据