首页|羧甲基壳聚糖诱导葡萄柚果实抗病防御代谢物差异分析

羧甲基壳聚糖诱导葡萄柚果实抗病防御代谢物差异分析

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
为研究羧甲基壳聚糖(CMCS)诱导采后葡萄柚果实抗性代谢物的差异,以葡萄柚果实为试材,采用1.5％CMCS和无菌水(CK)分别诱导葡萄柚24 h,利用非靶向代谢组学技术分析葡萄柚果实中代谢物的差异.结果表明,CMCS诱导葡萄柚24h,共得到92种差异代谢物,其中,上调36种,下调56种.KEGG通路注释结果表明,差异代谢物被显著(P＜0.05)富集到21条通路中,主要参与氨基酸和苯丙烷代谢.进一步筛选发现L-谷氨酰胺、L-谷氨酸、L-天冬酰胺、L-精氨酸、酪胺、丙二酸、莽草酸、D-赤藓-3-甲基苹果酸、反式肉桂酸和香豆素的表达量发生显著变化,且显著(P＜0.05)高于CK.另将相关差异代谢物与关键抗性差异基因(HSP17.4B、NCED1、GGCT2;2、HSP22.0、WRKY53、HSP22.0、WRKY40、WRKY54)进行相关性分析,结果证实两者之间存在显著正相关关系.
Analysis of Resistance and Defense Metabolites of Postharvest Citrus paradisi Induced by Carboxymethyl-chitosan
In order to study the difference of Carboxymethyl-chitosan(CMCS)-induced resistant metabolites inpostharvestgrapefruitfruits.Inthisstudy,grapefruitfruitswereusedastestmaterials,andgrapefruitwasinducedbyl.5％CMCS and sterile water(CK)for 24 h,respectively.The differences of metabolites in grapefruit fruits were ana-lyzed by non-targeted metabolomics.The results showed that a total of 92 differential metabolites were obtained after CMCS induced grapefruit for 24 h,of which 36 were up-regulated and 56 were down-regulated.The KEGG pathway annotation results showed that the differential metabolites were significantly(P＜0.05)enriched into 21 pathways,mainly involved in amino acid and phenylpropanoid metabolism.Further screening found that the ex-pression levels of L-Glutamine,L-Glutamic acid,L-Asparagine,Arginine,Tyramine,Malonic acid,Shikimic acid,D-erythro-3-Methylmalate,trans-Cinnamate and Coumarin changed significantly,and were significantly(P＜0.05)higher than CK.In addition,the related differential metabolites and key resistance differential genes(HSP17.4B,NCED1,GGCT2;2 HSP22.0,WRKY53,HSP22.0,WRKY40,WRKY54)confirmed that there was a significant positive correlation between the two.

Carboxymethyl-chitosanmetabolomeprevention and control after miningamino acid metabol-ismphenylpropane metabolism

刘胜红、杨清、吴化雨、唐勇、王芳、李根前、邓佳

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西南林业大学林学院,云南昆明 650233

西南地区生物多样性保育国家林业和草原局重点实验室,云南昆明 650233

西南山地森林资源保育与利用教育部重点实验室,云南昆明 650233

羧甲基壳聚糖 代谢组 采后防治 氨基酸代谢 苯丙烷代谢

国家自然科学基金国家自然科学基金云南省高层次人才培养支持计划青年拔尖人才专项云南省"两类"人才培养项目云南省农业联合专项

3196032632160394YNWR-QNBJ-2020-205202105AC160045202101BD070001-065

2024

10.11929/j.swfu.202306008

西南林业大学学报
西南林业大学

西南林业大学学报

CSTPCD北大核心
影响因子：0.773
ISSN：2095-1914
年,卷(期)：2024.44(3)
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