Effects of Combined Application of N,P and K on the Content of Endogenous Hormones of Leaves in the Seedlings of Osmanthus fragrans var.aurantiacus
According to an orthogonal experimental design using L9(33),3 factors(nitrogen,phosphorus,and potassium fertilizer)were set at 3 levels(N application rates of 0,5,and 10 g per plant;P2O5 application rates of 0,2,and 4 g per plant;KC1 application rates of 0,3,and 6 g per plant).The experiment on'Pucheng Dangui'seed-lings involved 2 rounds of nitrogen,phosphorus,and potassium combinations with a 30-day interval.The enzyme-linked immunosorbent assay(ELISA)method was used to measure the content of 4 hormones[indole-3-acetic acid(IAA),abscisic acid(ABA),gibberellic acid(GA3),and zeatin riboside(ZR)]in the leaves after 60,120,180,and 240 days of the second fertilization.The results showed that N5P0K3 treatment significantly increased the con-tent of IAA,ABA,and ZR,reaching their peak values at 240 days after fertilization(139.90,157.79,and 14.86 ng/g,respectively).Compared to the control group(CK),these levels increased by 101.52％,36.79％,and 73.08％,respectively.N5P2K6 treatment significantly increased the content of GA3,reaching its highest value(13.57 ng/g)at 240 days after fertilization,which was 97.71％higher than the CK group.N5P0K3 treatment significantly in-creased the ABA/GA3 and ZR/GA3 ratios,while significantly decreasing the ABA/IAA and ZR/IAA ratios.The effects of nitrogen and potassium factors on the content of IAA,GA3,ABA,and ZR differed significantly,while the phosphorus factor only showed a significant difference in the content of GA3.The combined application of ni-trogen,phosphorus,and potassium had a significant impact on the endogenous hormone content and homeostasis in the leaves of'Pucheng Dangui'seedlings.Based on variance and range analysis,the optimal fertilization scheme to increase leaf IAA,GA3,and ZR content was N5P2K6,while the optimal scheme to increase leaf ABA content was N5P0K3.
国家科技期刊平台
NSTL
万方数据
