卵叶远志内生真菌分离鉴定及乙酰胆碱酯酶抑制活性评价
Identification and AChE Inhibitory Activity of Culturable Endophytic Fungi Isolated from Polygala sibirica
申开泽 1胡祖茂 2尤梦吉 2罗丽花 2朱睿 2吴道秀 2华燕3
作者信息
- 1. 西南林业大学西南山地森林资源保育与利用教育部重点实验室,云南昆明 650233;昭通学院云南省天麻与真菌共生生物学重点实验室,云南昭通 657000
- 2. 昭通学院云南省天麻与真菌共生生物学重点实验室,云南昭通 657000
- 3. 西南林业大学西南山地森林资源保育与利用教育部重点实验室,云南昆明 650233
- 折叠
摘要
从云南双江采集的卵叶远志中分离可培养内生真菌,采用ITS测序对其进行鉴定,并对所得真菌的发酵提取物进行乙酰胆碱酯酶抑制活性筛选,以期探明卵叶远志内生真菌的多样性及其用于发掘治疗阿尔茨海默病先导化合物的潜力.结果表明:从卵叶远志中共分离到23株内生真菌,其中地上部分18株,根部5株,归属于1门4纲8目11科13属,优势属为刺盘孢属、镰刀菌属和附球酶属.采用Ellman法测定了内生真菌的甲醇提取物对乙酰胆碱酯酶的抑制活性,在测试浓度为0.5 mg/mL时,其中12株菌株表现出中等及以上强度的抑制作用(抑制率＞30％),Boeremia exigua PSA002、Fusarium oxysporum PSA008、Penicillium brefeldianum PSB007 等 3 株表现出较强的抑制作用(抑制率＞50％),其中,F.oxysporum PSA008的抑制率高达75.35％.采用UPLC-Q-TOF/MS对PSA002、PSA008和PSB007等3株真菌大米培养物的甲醇提取物进行分析,3个样品的质谱数据点响应强度和数量都较为明显且存在差异,且与大米培养基相比也均存在不同,表明真菌代谢转化形成了一定的化学多样性成分.本研究可为后续发掘卵叶远志相关活性化合物提供基础研究材料和理论参考.
Abstract
Endophytic fungi of Poly gala sibirica collected from Shuangjiang County of Yunnan Province were isolated and purified with traditional culture method,then these fungi were identified based on ITS se-quences.The fermentation extracts of the fungi were screened for acetylcholinesterase inhibitory activity to ex-plore the diversity of endophytic fungi in P.sibirica and their potential for treating Alzheimer's disease.The find-ings revealed that a total of 23 strains of endophytic fungi,including 18 from aerial tissues and 5 from the root of P.sibirica,were isolated.These fungi belonged to 13 genera,11 families,8 orders,4 classes and 1 phylum of Ascomycota.The dominant genera were Colletotrichum,Epicoccum and Fusarium.The inhibitory activities of methanol extracts of the 23 strains against acetylcholinesterase(AChE)were determined by modified Ellman's method.At a concentration of 0.5 mg/mL,12 of the strains showed moderate or high inhibitory effect against AChE(inhibition rate＞30％).The strain Fusarium oxysporum PSA008 exhibited the most potent inhibitory effect on AChE with inhibition rate of 75.35％,and other 2 strains(Boeremia exigua PSA002 and Penicillium brefeldian-um PSB007)also showed high AChE inhibitory activity(inhibition rate＞50％).UPLC-Q-TOF/MS was used to analyze the methanol extracts from rice culture of 3 strains of fungi,including PSA002,PSA008 and PSB007.The results showed that the response intensity and quantity of mass spectrum data points of the 3 samples were appar-ent and different,and compared with rice medium,there were also differences.The results indicated that the meta-bolic transformation of these fungi formed certain chemical diversity components.This study can provide basic research materials and a theoretical basis for further exploration of related compounds with AChE inhibitory activ-ity.
关键词
卵叶远志/内生真菌/真菌多样性/抗乙酰胆碱酯酶/阿尔茨海默病Key words
Polygala sibirica/culturable endophytic fungi/fungal diversity/annti-acetylcholinesterase(anti-AChE)/Alzheimer's disease(AD)引用本文复制引用
基金项目
国家重点研发计划项目(2021YFD1000202)
云南省地方本科高校基础研究联合专项资金项目(202110BA070001-059)
西南林业大学科技创新基金项目(KY21026)
出版年
2024
国家科技期刊平台
NSTL
万方数据