To comprehend the preference of codons in the chloroplast genome of Actinidia chinensis'Hongyang',51 coding Sequences(CDS)of A.chinensis'Hongyang'were analyzed to obtain to the results of Neutrality plot,ENC-plot and PR2-plot using Codon W and online software CUSP.The results showed that the GC content varied at different positions of the codons in the chloroplast genome of A.chinensis'Hongyang':Namely:GC(＞GC2＞GC3,indicating that the third base of codons preferred to end with A/U.The ENC value ranges from 35.70 to 56.79,indicating that codon usage bias in the chloroplast genome of A.chinensis'Hongyang'was generally weak.Neutrality plot,ENC plot and PR2 plot indicate that natural se-lection was the major factors on codon usage patterns in the chloroplast genome of A.chinensis'Hongyang'.In addition,17 codons were selected as the optimal codons and all of them ends with A/U.
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