LC-MS Analysis of Secondary Metabolites in the Callus of Vaccinium dunalianum
The investigation focused on the healthy callus of Vaccinium dunalianum,and the methanol con-tent was analyzed using LC-MS technology.The results showed that a total of 281 compounds divided into 14 categories were detected in the positive ion mode,which mainly including 58 terpenoids,41 alkaloids,28 organic acids,24 heterocyclic compounds,22 glycosides and 13 alcohols,with a relative content of 16.866％,13.711％,6.108％,10.138％,12.488％and 17.513％,respectively.A total of 71 compounds divided into 13 categories were detected in the negative ion mode,among which 14 alkaloids,9 organic acids,9 glycosides,7 terpenoids and 7 es-ters were the main components with a relative content of 5.839％,72.653％,3.423％,2.062％and 2.735％,respect-ively.Especially,the relative content of pyruvic acid was the highest at 47.474％.The results will provide a basis for the further regulation of the major chemical constituents in the callus of V dunalianum.
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