Melatonin alleviates zearalenone-induced oxidative stress and abnormal synthesis of estradiol in ovarian granulosa cells
The aim of this experiment was to study the toxic effects of zearalenone(ZEN)on ovarian granulosa cells and its effect on estradiol(E2)synthesis,and to provide a reference for the screening of drugs to alleviate the toxicity of ZEN.In the present study,human granulosa cell line(KGN)was utilized to establish a ZEN cell model.Cell viability was assessed using the CCK-8 method,while levels of reactive oxygen species(ROS)and mitochondrial membrane potential were measured using commercial kits.Additionally,mRNA expression levels of genes involved in E2 synthesis were determined through RT-qPCR a-nalysis.Simultaneously,compounds with potential alleviating effects on ZEN toxicity were screened and their rescue efficacy was validated.The results showed that the cell viability was significantly decreased with the increase of ZEN concentration(P<0.05).Meanwhile,the intracellular ROS level increased and the mitochondrial membrane potential level decreased significantly(P<0.05).Additionally,the mRNA expression levels of E2 synthesis genes CYP19A1,CYP11A1,STAR and HSD3B were sig-nificantly downregulated(P<0.05).Compared with the ZEN treatment group,the combined addition of melatonin(MT)could increase the number of living cells and significantly improve the cell viability(P<0.05).The mRNA expression levels of E2 synthesis genes CYP19A1,CYP11A1,STAR and HSD3B were significantly upregulated(P<0.05),and the intracellular ROS level and cell membrane potential level were significantly restored.In summary,ZEN caused oxidative stress and disordered E2 synthesis in KGN cells,while MT partially alleviated the above toxic symptoms,suggesting that MT could be used to alleviate the toxicity of ZEN.
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