Effects of selenium and vitamin E injected into eggs on antioxidant and immune performance of 1-day-old broiler chickens
To investigate the effects of selenium and vitamin E injected into embryonic eggs on antioxidant and immune perform-ance of 1-day-old chickens,240 yellow-feathered broiler eggs with similar weight were selected and randomly divided into 4 groups:The control group(normal saline),selenium group(20 mg selenium per egg),VE group(5 mg vitamin E per egg)and mixed group(20 mg selenium+5 mg vitamin E per egg)were incubated to 10 embryo age,and four different substances were injected into the yolk sac of the embryonic eggs.After hatching,the chickens were counted and the related indexes of 1-day-old broilers were detected.The results showed that compared with the control group,the breast muscle index and liver index of broil-ers at 1 day of age significantly increased in the mixed group(P＜0.05),the spleen index significantly increased in the three experimental groups(P＜0.05);The activities of serum catalase(CAT),serum glutathione peroxidase(GSH-Px)in VE group,serum glutathione peroxidase(GSH-Px)in selenium group and serum CAT,GSH-Px and superoxide dismutase(SOD)in mixed group significantly increased(P＜0.05);In liver,the activity of GSH-Px in VE group and SOD in Se group signifi-cantly increased(P＜0.05).;Liver expression levels of CAT,SOD,GSH-Px,nuclear factor erythrocyte line 2 associated factor 2(Nrf2),tumor necrosis factor-α(TNF-α)and interferon-γ(IFN-γ)in VE group were significantly up-regulated(P＜0.05),the gene expressions of CAT,Nrf2 and interleukin-1 β(IL-1β)in liver of mixed group were significantly up-regulated(P＜0.05).The results showed that selenium and VE injected in eggs significantly improved the antioxidant and immune prop-erties of 1-day-old broilers,and promoted the growth and development of tissues and organs of chicks.The effect of selenium and VE combined injection was better than that of individual injection.This study provided a scientific basis for the early application of selenium and VE to chicken embryos to improve the antioxidant and immune properties of chickens.
selenomethioninevitamin Ein ovo injectionanti-oxidationimmunitygene expression
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