Molecular characteristics of MYOZ2 gene and its expression in leg muscles of Luning chickens at different ages
The aim of the research was to investigate the molecular characteristics of the MYOZ2 gene in male Luning chickens,its expression profiling in different tissues and expression levels in leg muscles at different developmental stages,and to explore the effect of MYOZ2 gene on the growth and development of Luning chickens.The coding sequence(CDS)of MYOZ2 gene was obtained by reverse transcription-polymerase chain reaction(RT-PCR)and analysed by bioinformatics.Quantitative polymerase chain reaction(qPCR)was used to detect the differential expression of MYOZ2 gene in heart,liver,spleen,lung,kidney,pecto-ral and leg muscle tissues of Luning chickens,as well as in leg muscles at different stages of development.The results revealed that the open reading frame(ORF)of MYOZ2 gene was 792 bp in length,encoding 263 amino acids;the MYOZ2 protein was predominantly located in the nucleus,with the secondary structure showing a high proportion of random coils.The nucleotide se-quences of Luning chicken MYOZ2 were compared with those of other species,including proto chickens,turkeys,guinea fowls,hummingbirds,rainbirds,sparrows,Chinese hamsters,cows,goats,pigs and human beings,demonstrating 100％homology with the original chicken sequence.Notably,a lower degree of sequence similarity was observed with humans,Chinese hamsters,cows,sheep,and pigs,indicating a higher evolutionary conservation of the MYOZ2 gene within the same species.Tissue expres-sion analysis indicated that the expression of MYOZ2 in the heart was significantly higher compared to other tissues,except for the spleen(P＜0.05);and the MYOZ2 gene was expressed in the leg muscle tissues of the 20 d,36 d,51 d,and 94 d post-hatch.The MYOZ2 gene expression was the highest at 20 d,which was significantly higher(P＜0.05)than 51 d and 94 d post-hatch.This study laid a foundation for a deeper understanding the role of MYOZ2 gene in the regulation of growth and develop-ment and other physiological functions in male Luning chickens.
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万方数据
