Study on the expression of G6PD in the major reproductive organs of female yaks during different reproductive cycles
The object of the study was to explore the expression of G6PD in the main reproductive organs of yaks in different re-productive cycles,and to provide a theoretical basis for further understanding the role played by G6PD in the reproductive process of yaks.The relative expression of the G6PD gene in the ovary,fallopian tube and uterus of yak during follicular,luteal and pregnancy was detected by PCR and qRT-PCR;the relative expression of G6PD protein in the ovary,fallopian tube and ute-rus was detected by Western blotting(WB).The results showed that the G6PD was expressed in all major reproductive organs of yaks,and the expression on the ovary in the luteal phase was significantly higher than that in the follicular phase(P＜0.05);G6PD in the fallopian tube in pregnancy was considerably higher than that in the luteal phase and follicular phase(P＜0.05),and in the uterus during pregnancy was higher than that in the follicular phase and luteal phase.The results indicated that G6PD played an important role in the main reproductive organs of yaks in different reproductive cycles,and provided refer-ence materials for further research on the mechanism of G6PD in mammalian reproduction.
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