Antibiotic resistance profiles of Escherichia coli and Enterococcus spp.originated from pet clinics and trading markets in Chengdu City
To investigate the antibiotic resistance status of Escherichia coli and Enterococcus spp.in the pet clinic and trading market environments in Chengdu City,this study collected 433 samples(98 air samples and 335 surface samples from various objects)from 15 pet public activity sites and identified 174 E.coli strains and 288 Enterococci strains by bacterial culture,dif-ferentiation,and 16S rRNA gene sequencing.The results showed that more target bacteria could be isolated from environments with poor cleaning conditions.The antibiotic resistance feature analysis showed that 163 E.coli strains and 288 Enterococci strains were resistant to beta-lactam,tetracycline,and fluoroquinolone antibiotics,respectively.Among the resistant Escherichia coli strains,the proportion of double resistant strains was the highest(41.95％,73/174),and 65 strains were multi-resistant(7.35％,65/174),with cefotaxime-doxycycline-enrofloxacin as the main resistant phenotype(44.62％,29/65).The main phenotype of resistant Enterococcus was the dual resistance to doxycycline and enrofloxacin(38.54％,111/288),and 56 strains were multi-resistant bacteria(19.44％,55/288),among which ampicillin-amoxicillin/clavulanate-doxycycline-enroflox-acin was the representative resistance phenotype(94.64％,53/56).The majority of the resistant bacteria were isolated from the environments of pet clinics,and showed diversity in the antibiotic-resistant phenotypes.The dominant resistance genes carried by multi-drug resistant E.coli were tetA(92.31％,60/65),tetB(100％,65/65),blaTEM(75.38％,49/65),and blaCTX-M(70.76％,46/65),while the dominant resistance gene carried by multi-drug resistant Enterococci was blaTEM(41.82％,23/55).In summary,the results of this study clearly indicated the prevalence of bacterial resistance to commonly used antibiotics o-riginated from different pet activity environments in Chegndu City,which carried abundant resistance genes,revealing the risks of antibiotic resistance transmission and spread between human-pet populations,and providing perspectives for future research on the formation of environmental bacterial resistance and monitoring measures of public health significance.
Chengdu Citypet service locationEscherichia coliEnterococcus spp.antibiotic resistance
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