A study on the improvement of the method for paraffin sectioning of dried herbs from different parts of the medicinal plant
Paraffin sectioning is widely used in histology as one of the key techniques for microscopic identification of dried herbs.According to the characteristics of different tissue structures,representative,stem,seed and root dry herbs were selected as the main experimental materials,and the traditional preparation methods were improved mainly from softening,dehydration and transparency to explore the exclusive paraffin preparation methods for each herb.The results showed that softening is the key step in making paraffin sections,and different kinds of dried herbs should be treated differently.Cortex herbs enriched with fiber and stone cells,such as Magnolia officinalis,were softened with 5％hydrofluoric acid in a water bath for 12 h;stems herbs with severe xylem fibrosis,such as Cinnamomum cassia,were softened for 14 h using a mixture of glycerol and sodium hydroxide;seeds of herbs with large differences in internal and external hardness,such as Sinapis alba,were softened with 5％glycerol for 6 h;roots and stems with strong fibers and reagents that were not easy to penetrate,such as Astragalus membranaceus,were sof-tened with 5％hydrochloric acid for 6 h,which could improve the clarity and completeness of the cross section of each dried herb,and obtain high-quality slices with complete tissues and clear cellular structures.The quality of sections with complete or-ganization and clear cellular structure was obtained.The modified paraffin sectioning technique was highly specific and repro-ducible,avoiding the problems of incomplete wax dipping and hardening and brittleness of tissues,which are prone to occur in conventional sectioning.The results of this study could provide a methodological reference for the production of paraffin sections of different parts of the tissue.
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