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三株乳酸菌混合发酵对发酵乳品质影响分析

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为探究不同乳酸菌菌株混合发酵对发酵乳品质影响,选用植物乳植杆菌、嗜热链球菌、乳酸乳球菌为研究对象,通过单菌发酵和混菌发酵对发酵乳酸度、质构特性、感官评价等进行探索,并采用气相色谱-质谱联用(gas chroma-tography-mass spectrometry,GC-MS)对发酵乳进行风味成分分析。结果显示,相同发酵时间下混合发酵组的酸度上升更快,混合发酵组的质构特性及感官特性比单菌发酵组更好,混菌发酵酸乳较单菌发酵组口感更加粘稠;GC-MS分析结果表明混菌发酵组独有的风味化合物有4种,分别为乙醇、1-戊醇、β-蒎烯、环辛四烯;通过相对气味活度值(relative odor activity value,ROAV)对混菌发酵组和单菌发酵组挥发性成分进行分析,鉴定出3种关键风味物质,分别为桉叶油醇、芳樟醇、(-)-4-萜品醇,对酸乳的风味形成影响较大。结果表明,混菌发酵对发酵乳质构特性和感官及风味特性影响较大,为后期菌株的工业化应用提供理论依据。
Effect of mixed fermentation by three strains of lactic acid bacteria on the quality of fermented milk
To investigate the impact of mixed fermentation by different strains of lactic acid bacteria on the quality of fermented milk,this study selected Lactiplantibacillus plantarum,Streptococcus thermophilus,and Lactococcus lactis as the research sub-jects.Exploration was conducted on the fermentation lactic acid degree,texture characteristics,and sensory evaluation through both single-strain and mixed-strain fermentations.Gas chromatography-mass spectrometry(GC-MS)was used for flavor compo-nent analysis of the fermented milk.The results showed that under the same fermentation time,the acidity of the mixed fermenta-tion group increased more rapidly,and the texture and sensory characteristics of the mixed fermentation group were better than those of the single-strain fermentation group.The yogurt from mixed-strain fermentation was more viscous in mouthfeel compared to the single-strain fermentation group.The GC-MS analysis indicated that there were four flavor compounds in the mixed-strain fermentation group,which are ethanol,1-pentanol,β-pinene,and cyclooctatetraene.By analyzing the volatile components of both mixed and single-strain fermented milk using the relative odor activity value(RO AV),three key flavor substances(eucalyptol,linalool,and(-)-4-terpineol)were identified,which had a great impact on the flavor formation of yoghurt.The results of this study indicated that mixed fermentation had a significant effect on the texture,sensory,and flavor characteristics of fermented milk,providing a theoretical basis for the future industrial application of these strains.

lactic acid bacteriamixed fermentationfermented milk qualitytexture property

冶海潇、杨洋洋、王金丽、朱成林、陈娟、唐俊妮

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西南民族大学药学与食品学院,四川成都 610225

乳酸菌 混合发酵 发酵乳品质 质构特性

2024

西南民族大学学报(自然科学版)
西南民族大学

西南民族大学学报(自然科学版)

CSTPCD
影响因子:0.441
ISSN:2095-4271
年,卷(期):2024.50(6)