Genome sequencing and antimicrobial substance gene analysis of Bacillus pumilus from yak
The aim was to analyze the genome sequence of Bacillus pumilus SMU5927 to excavate the functional genes of the strain.The DNA of the strain was extracted,and the library was established and checked on HiSeq platform.The strain was se-quenced and analyzed on Novaseq 6000 sequencing platform,and the gene function was annotated with GO,KEGG,COG and NR databases respectively.The total genome length of SMU5927 was 5 502 707 bp,the GC content was 40.98％,and the total length of the encoded gene was 4 788 108 bp.GO gene function annotation analysis of the strain showed that 5 genes were in-volved in immune process,2 genes were related to cell proliferation and 11 genes were related to antioxidant activity in SMU5927.There were 3 440 genes annotated in KEGG database,and 6 genes were related to immunity,and there were 5 genes related to PPAR signaling pathway.Forty-six genes in COG were related to MFS transporter,which ensured the tolerance proper-ties of SMU5927.Through NR analysis,it was found that the genome contained 33 genes related to potential antibacterial sub-stances.Surfactin,cyclic bacteriocin and Holin played the role of pathogen resistance and bacteriostasis.MrsA,MsrB,PDF,NRPSs and MraY had the potential to promote the production of antibiotics and made antibacterial agents.SppA,RF1,RF2,and RF3 could promote the release of proteins.SOD,CAT,and SCO family proteins could defend against oxidative stress.In conclu-sion,through the analysis of the genome sequence of SMU5927,it was further verified that there were antibacterial substances in Bacillus pumilus at the molecular level.This study validated an important theoretical basis for the exploration of its function and antibacterial mechanism,which provided theoretical basis and scientific guidance for SMU5927 as a substitute for antibiotics.
yakBacillus pumiluswhole-genome sequencinggene function annotationantimicrobial substance
NETL
NSTL
万方数据
