Optimization of formulation and preparation for hydroxypropyl tetrahydropyrantriol liposomes using Box-Behnken response surface method
In order to improve the skin permeability of hydroxypropyl tetrahydropyrantriol,liposomes were made,and the formu-lation and preparation process were optimized.Using the size and encapsulation rate of the liposomes as indicator,reverse evapo-ration method was used as the preparation method of hydroxypropyl tetrahydropyrantriol liposomes.Based on data of previous single-factor investigation,the ratio of phospholipid to cholesterol,drug-lipid ratio and ultrasound time,which had a significant impact on the evaluation indexes,were selected as the objects of investigation,and the size and encapsulation rate of liposome were taken as the indexes.The Box-Behnken response surface method was used to optimize the formulation.The following opti-mal formula was obtained:phospholipid to cholesterol ratio of 4.49∶1,drug to lipid ratio of 0.23∶1,and ultrasound time of 7.2 minutes.The prepared liposomes had good appearance,with average particle size(93.46±11.81)nm,encapsulation rate(67.10±1.83)％,and drug loading(11.15±0.30)％.
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