Detection of Catalytic Characteristics of Polyphenol Oxidase in Lotus Root
In this trial , the PPO in lotus root was extracted by ginding with ice-bath and centrifugal method , and the effects of pH , temperature. concentration of aubatrate. and inhibitors on PPO's activiy were investigated by using the spectrophotometric method. The results showed that the optimal pH and tempearture were 7. 0 and 20 ℃ , Km and Vmax of PPO with catechol aubstrate were 0. 18 mol/L and 0. 900 OD/min, resppectively; sodium sulfite and vitamin C were strong inhibitors;the PPO in lotus root had the aubatrate specificity.
NETL
NSTL
万方数据
维普
