Effects of Different in vitro Pollination Methods on Capsule Formation in Lilium Hybridization
To overcome incompatibility caused by pre-fertilization barriers in lily breeding, cultivars from five series of Lilium hybrids and one wild species as experimental materials , different in vitro pollination methods such as in vitro stigmatie pollination ,in vitro cut-atyle pollination and in vitro ovarian pollination were uaed to cany out the hybndization. The result, showed that in intrsspecific crosses , percentages of awollen ovaries and shaped capsules were 30 ％ and 15 ％ for in vitro stigmatic pollination, 15 ％ and 5 ％ for in vitro eut-style pollination. 25 ％ and 5 ％ for in vitro ovarian pollination. In interspecific crosses, percentages of swollen ovaries and shaped capsulea for the three in vitro pollination methods were 17 ％ and 13 ％ , 3 ％ and 3 ％ , 10 ％ and 3 ％ , respectively. Hybridization type had a great effect on capsule fonnation; Percentages of awollen ovaries and shaped capsules both were higher in intraspecifie crosses than in interspecific crosses. In vitro stigmatic pollination had the best effect for overcoming the pre-fertilization harrier in lily hybridization ; the other two methods showed less and same effectiveness. Hybrid seedlings of some combinations were obtained by virtue of embryo rescue and identification of the hybrids was accomplished with ISSR markers, which verified the truth to obtain lily hybrids of interspecific crosses using in vitro pollination technique.
NETL
NSTL
万方数据
维普
