Study on Characteristics of in Situ Pollen Germination and Tube Growth of Lycium barbarum L.
The self-and cross-pollination behavoirs of 3 Lycium barbaum Ningqi1, Ningqi3, Baihua were observed by fluorescence microscopy. The results showed that the pollen tube of self- pollination Ningqil and cross-pollination combination could enter the germen from 12 to 24 h and integrate with the ovule, and achieve fecundation in 48 h. The germination time of self-pollination Ningqi3 and Baihua was longer than that of self-pollination Ningqi1, and its growth speed and stop position of pollen tube in style were different. The pollen tubes of Baihua grew slowly and stoped in stylar basal,but the pollen tube growth of Ningqi3 grew quickly and entered the germen. The pollen tube growth of cross-pollination combination was quicker than that of self-pollination Ningqi1, which was consistent with the investigation in field.