The main chemical components of X2F, C3F, B2F leaves of flue-cured tobacco K326 from 19 tobacco regions of Yunnan province were analyzed and comprehensively evaluated. The result indicated that the average chemical components of flue-cured tobacco samples in Yunnan were almost in normal range of high quality flue-cured tobacco, which indicated that the flue-cured tobacco in Yunnan had appropriate content of chemical components, and had high quality. By systematically clustering on C3F, the tobacco regions of Yunnan were divided into four sub-regions of high chlorin- low potassium areas, high potassium- low nicotine areas, high sugar- low chlorin areas, and high nicotine- low sugar areas. The quality of tobacco leaves of the second and third sub-regions performed better, which included Xinpin county, Gengma county, Malong county, Jingdong county and Lufeng county.