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云南茶树资源儿茶素和没食子酸的差异性分析

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以勐海国家种质茶树资源圃中的121个云南茶树资源为材料,采用高效液相色谱法(HPLC)测定茶叶中的没食子酸(GA)、儿茶素(C)、表儿茶素(EC)、表儿茶素没食子酸酯(ECG)、表没食子酸儿茶素(EGC)及表没食子儿茶素没食子酸酯(E(GCG)的含量.结果表明,GA含量变幅为0.210% ~1.902%,平均0.834%,在茶树中含量较低.C含量变幅为0.069% ~8.865%,平均1.916%;EC含量变幅为0.126% ~2.865%,平均1.112%;EGC含量变幅为0.00% ~3.709%,平均0.954%;ECG的含量变幅为0.739% ~8.957%,平均4.063%;EGCG含量变幅为0.819% ~11.77%,平均5.939%;儿茶素总量含量为6.354% ~22.654%,平均14.042%.资源间儿茶素差异较大,说明云南茶树资源具有丰富的生物多样性.同时,筛选出3份高EGCG含量(≥1O%)的特异茶树资源,为高EGCG茶树品种选育提供了重要材料.
Difference Analysis on Catechins and Gallic Acid Contents of Tea Resources from Yunnan Province
By using high performance liquid chromatography (HPLC), the gallic acid(GA), catechins(C), epicatechin(EC) , epigallocatechin( EGC) , epicatechin gallate( ECG) and epigallocatechin gallate ( EGCG) contents of 121 tea germplasms from the China National Germplasm Tea Repositories (CNGTR) at the Tea Research Institute of Yunnan Academy of Agricultural Sciences (TRIYAAS) were measured. The results showed that the content of GA ranged from 0.210 % to 1.902 %, with an average of 0.834 % , explaining relatively low GA content among tea germplasms; the content of C ranged from 0.069 % to 8.865 % , with an average of 1.916 %; the content of EC ranged from 0.126 % to 2.865 %, with an average of 1.112 % ; the content of EGC ranged from 0.00 to 3.709 %, with an average of 0. 954%; the content of ECG ranged bom 0.739% to 8.957%, with an average of 4.063 %; the content of EGCG ranged from 0.819 % to 11.77 % , with an average of 5.939 % ; and the content of total C ranged from 6.354 % to 22.654 % , with an average of 14.042 %. There was relatively big difference of catechin contents among different tea resources, indicating that there was plentiful biodiversity of Yunnan tea gennplasms. At the same time, three tea germplasms with high epigallocatechin gallate content (≥10 % ) was selected preliminarily, which would provide important materials for breeding tea cultivars with high EGCG content in the future.

Yunnan provinceTea germplasmGallic acidCatechinsDifference

刘本英、宋维希、马玲、段志芬、蒋会兵、孙雪梅、黄玫、矣兵、汪云刚、王平盛

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云南省农业科学院茶叶研究所,云南勐海666201

云南 茶树资源 没食子酸 儿茶素 差异性

国家现代农业(茶叶)产业技术体系建设专项资金国家自然科学基金云南省农科院茶叶所所列项目农业部保种项目云南省科技创新人才计划

nycytx-23311601752009A0937NB20112130135-012011CI068

2012

西南农业学报
四川,云南,贵州,广西,西藏及重庆省(区,市)农科院

西南农业学报

CSTPCDCSCD北大核心
影响因子:0.679
ISSN:1001-4829
年,卷(期):2012.25(3)
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