The influencing factors were studied in the fast breeding system and the inducement process of callus for Ligusticum chuanxiong in this paper. The results showed that April was the best season to get experimental stuff of Ligusticum chuanxiong for tissue culture; the effect of NAA was better than that of 2,4-D; the main factors were sucrose and 6-BA in the inducement process of stem node callus; the best inducement culture medium was MS + 1.5 mg/LNAA +0.5 mg/L 6-BA + 40 g/L sucrose, and the best differentiation culture medium was MS + 2.0 mg/L 6-BA +0.2 mg/L NAA +20 g/L sucrose; sucrose was better than glucose in the carbon sources; the best organic additions was 450 mg/L CH; the best rooting medium was 1/2MS +0.5mg/LNAA + 30g/L sucrose.
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NSTL
维普
万方数据
