Identification of Tobacco Endophytic Bacterium ZY-9-13 and Its Antifungal Activities against Phytopathogens
Species of tobacco endophytes are abundant.Recently, more and more attention was focused on these resources as bicontrol agents against phytopatogens.In this study, ZY-9-13, a bacterial endophyte isolated from leaf of tobacco variety K326, showed a wide range of antifungal spectrum which exhibited antagonistic activities towards all the 6 tested phytopathogens.The antifungal activities of ZY-9-13 against the mycelial growth of Phytophthora parasitica var.nicotianae,Fusarium oxysporum f.sp.cuben,F.solani,F.omysporum f.sp.cucumerinum,Gibberella sanbinetti and Rhizoaonia solani were 68.13 ％, 68.53 ％, 52.23 ％, 46.61％, 57.85 ％ and 43.45 ％, respectively.ZY-9-13 could cause the mycelia and conidia of fungi into deformation and diapause.Based on the morphological, physiological and biochemical characteristics, as well as phylogenetic analysis of 16S rRNA gene sequence, ZY-9-13 was identified as Bacillus amyloliquefaciens.
Bacillus amyloliquefaciensEndophytic bacteria of tobaccoBiological control
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