Fungal Communities Diversity in Salviae Miltiorrhizae Planting Rhizospheric and Non-rhizospheric Soil Analyzed by PCR-DGGE in Zhongjiang, Sichuan Province
Using non-rhizospheric and rhizospheric soil of sick and normal growing Salvia miltiorrhiza as materials,the soil physicochemical properties and soil fungal diversity was determined.The result showed that,compared with the normal growth of Salvia miltiorrhiza,the organic matter content,total nitrogen,alkali-hydrolysis nitrogen,available phosphorus and available potassium content of was decreased by 60 ％,52 ％,18 ％,18 ％ and 45 ％ in the soil of sick Salvia miltiorrhiza.PCR-DGGE analysis showed that the fungal groups in rhizospheric and non-rhizospheric soil of sick Salia miltiorrhiza were significant differences.Sequencing results of representative cloned DGGE bands showed that the predominant groups belonged to Acremonium sclerotigenum,Phoma sp.,Phialocephala fortiniiand Chaetomium sp..The results suggested that soil fertility decrease and pathogenic fungi increase leads to the continuous cropping obstacle of Salvia miltiorrhiza.
Salvia miltiorrhiza BgeContinuous cropping obstaclesFungal diversitySoil physicochemical properties
NETL
NSTL
万方数据
维普
