A method for the determination of 8 organic acids in flue-cured tobacco seeds with gas chromatography was devdoped.Samples were derivatizated by 10 ％ sulfuric acid-methanol (v/v),extracted by dichlonomthame,determined by gas chromatography (FID) detector.It was found that the recoveries of the method was between 98.6 ％ and 100.9 ％,the relative standari deviation was equal or less than 3.3 ％,the correlation coefficients of linear regression equatiors were from 0.9991 to 0.9998.The content of 8 organic acids in 13 tobacco seeds samples were determined with this method,and the data were processed with cluster analysis.Results showed that the 13 samples could be dassified into 4 classes,the proportion distribution of 8 organic scids reflected dearly the charactedstics of different flue-cured tobacco seeds,which could be served as a reference in tobacco seeds classification.
NETL
NSTL
万方数据
维普
