首页|干旱胁迫下不同植物生长调节剂对烟草幼苗抗逆性的影响

干旱胁迫下不同植物生长调节剂对烟草幼苗抗逆性的影响

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应用2,7-二氮荧光素(DCFH)染色方法定性检测烟草幼苗在不同干旱条件下叶片中活性氧自由基(AOS)的变化,并与对照组比较,分析不同植物生长调节剂对烟草幼苗抗旱性的影响.通过实验发现,与对照组比较,持续干旱处理组叶脉中AOS的积累随干旱程度加深而增加.植物生长调节剂(6-BA、2,4-D、IBA)处理可以从一定程度上提高烟草幼苗在干旱胁迫下的抗逆性,其中2,4-D的作用最显著,6-BA次之,而IBA的作用效果则不明显.
Effects of Different Phytohormones on Resistance of Tobacco Seedling under Drought Stress
2,7-dichloro-fluorescein (DCFH) staining method was used to qualitativdy detect the levels of Reactive Oxygen Species (AOS)in tobacco leaves under different drought conditions with application of different exogenous phytohormones (6-BA,2,4-D,IBA).The experiment revealed that persistent drought stress would induce the accumulation of AOS in leaf veins and the degree would increase accompanied by the level of drought stress.In addition,exogenetic phytohormones could increase the resistance of tobacco seedling to the drought stress to some extent.2,4-D had the greatest effect,followed by 6-BA.The IBA treatment did not show obvious influence.

Plant hormonesDroughtResistanceDCFH staining

杨波、苏鸿雁、刘硕然

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大理学院农学与生物科学学院,云南大理671003

植物激素 干旱 抗性 DCFH染色

云南省高校滇西生物多样性科技创新团队基金资助项目大理学院质量工程项目

云教函[2011]93号TD1-05

2014

西南农业学报
四川,云南,贵州,广西,西藏及重庆省(区,市)农科院

西南农业学报

CSTPCDCSCD北大核心
影响因子:0.679
ISSN:1001-4829
年,卷(期):2014.27(6)
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