Focusing on high throughput SSR markers development based on the genome synteny between woodland strawberry (Fragaria yesca) with roses,we employed Microsatellite software to scan the woodland strawberry genome sequence with 206.8 Mb length,total 49 029 SSR loci were identified and the average distance between SSRs was 4.2 kb.21288 SSR primer pairs were successful designed by primer 3 software.Then,we selected 300 primer pairs to validate the transferability to rose genome using Rosa chinensis ‘ Old blush’ and Rosa wichuriana ‘ Basye' s thornless’.As a results,forty SSR microsatellite loci successful amplified,and the rate of amplification was 13.3 percent.In additional,we found a 63 959 bp high conservative genome region contains eight transferable SSR loci.The work present here would provide a molecular evidence for comparative genome research between Fragaria vesca with roses and a larger set of SSR markers.
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维普
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