For the purpose of genetic transformation and provides the theoretical foundation of molecular breeding for the subsequent varieties of Yuzi 7,the authors adopted the strategy of homogenous clone and the method of RACE techniques to clone its key enzyme genes in the biosynthetic pathway and analyze it in bioinformatics.Results:The full-length cDNA of flavanone 3-hydroxylase (F3H)was cloned from Yuzi 7,(GenBank accession number:KU144881),the full length of sequence was 1280 bp,including 5' terminal UTR,a coding region which encoded 368 amino acid residues,3' terminal UTR and a polyA tail;F3H protein has combined with Oxoglutarates to get a RXS motif Arg287-Ser285 and combined with the ferrous ion (Fe2+) to get conserved amino acids His219,Aps221 and His277.Compared F3H of Yuzi 7 with F3H of Ipomoea quamoclit in GenBank,the gene sequence has a high similarity,96 ％.The proteins of Yuzi 7 F3H and Ipomoea quamoclit F3H in related species has a high degree of similarity in amino acid,94 ％.The F3H cDNAs cloned from Yuzi 7 conforms to the ubiquitous F3H genes in plants and it has biological activity.
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