首页|草莓根部侵染草莓炭疽菌的致病动态变化过程

草莓根部侵染草莓炭疽菌的致病动态变化过程

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为了从组织学和细胞学水平上了解草莓炭疽菌(Colletotrichum fragariae)病原菌致病机理及其与寄主的互作机制,通过草莓炭疽菌分生孢子液接种侵染健康草莓幼苗根,在荧光显微镜下观察病原菌的侵染过程,研究草莓根部浸染草莓炭疽菌的致病性.结果表明,接种12 h,95％的分生孢子萌发;接种24h,69％芽管顶端产生附着胞或附着技并形成侵入钉开始便染根表皮细胞;接种48h,产生大量菌丝并纠结成网状;接种4d,少量的根尖开始变褐;接种9d,整个根系几乎变为黑褐色,产生少量分生孢子盘;接种13 d,侵染菌丝扩展到主轴根微管柱,新的分生孢子产生,完成一个侵染循环;接种20 d,侵染菌丝入侵到达叶柄和花茎;接种41 d,侵染菌丝扩散到叶柄表面并便染叶柄腺毛;接种55d,便染菌丝入侵到达叶片并便染叶片腺毛,组织细胞开始崩解死亡,叶片上形成典型的炭疽病病斑.[结论]:C.fragariae能通过根便染到整株植株,属于系统性便染.
Pathogenic Process of Strawberry Roots Infected by Colletotrichum fragariae
In order to know the pathogenesis of Colletotrichum fragariae and the interaction mechanism with the hosts at levels of histology and cytology,the healthy strawberry seedlings were inoculated with conidla of pathogen C.fragariae by root dipping in conidia suspension.The pathogen infection process was observed by fluorescence microscope at different time points after inoculation.Results:After 12 h,95 ％ of conidia germinated,about 69 ％ conidia formed classical appressoria and hyphopodia,and infection pegs began to penetrae the epidermal cells of roots at 24 h,and by 48 h,the hyphae had extensively colonized and formed a network around the roots.By four days,several plants had developed slightly brown discolouration at root tipe,by nine days,almost all roots had developed largely black and/or brown discolouration and formed a spot of acervulus.By 13 days,hyphae had invaded to the vascular bundle on the main roots and formed the new conidia,completing the disease cycle.By 20 days,hyphae had invaded petioles and rachis above ground,and were found on the petioles surface and glandular hairs of petioles at 41 d.By 55 d hyphae had invaded the leaves and their glandular hairs.Typical anthracnose disease spots were found on the leaves,which had begun to decompose.Conclusion:subsequent to inoculation,the C.fragariae infection can spread across the entire strawberry plants via the roots,which is indicative of a systemic infection.

Colletotrichum fragariaeStrawberry anthracnoseInfection processColonizationAppressoriaHyphopodiaFlureceneeFluorescence microscope

赵玳琳、赵兴丽、卯婷婷、张敬泽、蔡磊、陶刚

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贵州省植物保护研究所,贵州贵阳550006

贵州师范大学,贵州贵阳550000

浙江大学,浙江杭州310058

中国科学院微生物研究所真菌学国家重点实验室,北京100101

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Colletotrichum fragariae 草莓炭疽病 侵染过程 定殖 附着胞 附着枝 荧光显微镜

贵州省优秀青年科技人才培养对象专项资金贵州省农业科学院专项贵州省农科院自主创新能力项目

黔科合人字2015272012022黔农科院自主创新科研专项字2014002

2016

10.16213/j.cnki.scjas.2016.03.021

西南农业学报
四川,云南,贵州,广西,西藏及重庆省（区,市）农科院

西南农业学报

CSTPCDCSCD北大核心
影响因子：0.679
ISSN：1001-4829
年,卷(期)：2016.29(3)
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