Spot canker survey,pathogen isolation and identification of Eucalyptus in several cities of Guangxi such as Nanning,Chongzuo,Qinzhou,Wuzhou,Yulin and Hechi were performed.The pathogen was identified through morphological characteristics and genetic molecular analysis,which could lay an etiology foundation for preventing and treating Eucalyptus canker.The results showed that the pathogen causing canker was Cytospora eucalypticola,Neofusicoccum parvum and Lasiodiplodia theobromae,of which Lasiodiplodia theobromae was a newly-found canker pathogen.