The genetic diversity of 18 phenotype characters of 53 Frutescens germplasm resources was analyzed by the clustering and principal component analysis methods to provide the scientific basis for identification,taxonomy and genetic improvement application of Perilla Frutescens germplasm resources in Guizhou.Results:There is wide genetic diversity in Frutescens germplasm resources and are significant positive correlations or negative correlations between different characters of Frutescens germplasm resources.The variation coefficient of six quantitative characters of 53 Frutescens germplasm resources is 24.12 %-66.49 %.The variation coefficient of total panicles per plant and nodes of main stem is maximum (66.49 %) and minimum (24.12 %) respectively.53 Frutescens germplasm resources are divided into three groups by clustering analysis based on different phenotype.Group Ⅰ belongs to the ornamental type with late maturity,umbrella plant type,deep leaf color and less seed weight per plant,group Ⅱ is of early maturity,tower plant type and big seed and group Ⅲ is the middle group between early maturity type and late maturity type,which reflects the genetic relationship among three groups basically.The principal component analysis shows that the accumulative contribution rate of former 7 principal components is up to 84.79 %.The load value of different principal components reflects the breeding selection potential of major characters.