Secondary Metabolites from Houttuynia cordata Thunb.and Their Antibacterial Activities
[Objective] To analyze and identify the chemical composition of volatile oil extracted from Houttuynia cordata,the inhibition activities of volatile oil and ethyl acetate extracts against 7 different bacteria were determined.[Method] The volatile oil from Houttuynia cordata was extracted by hydro-distillation,and the ethyl acetate extracts was extracted by methanol extraction at room temperature.Gas chromatography-mass spectrometry (GC-MS) was used to analyze the chemical compositions of the essential oil,and inhibition zone method and TLC-MTT-bioautography assay were used to detect the inhibitory activities of the volatile oil and ethyl acetate extracts against bacteria,respectively.[Result]The essential oil from fresh weight basis of Houttuynia cordata was extracted with the yield of 0.09 ％,and forty-nine components from the volatile oil were identified,which accounted for 93.35 ％ of total contents,of whichβ3-Pinene (23.65 ％),2-Tridecanone (12.38 ％),2-Undecanone (8.55 ％),Bornyl acetate (4.08 ％),Dodecanoic acid (3.77 ％),1-Tridecene (3.51％),Caryophyllene oxide (2.97 ％) and α-Cyperene (2.59 ％) were the major compounds.The volatile oil had certain antibacterial activities on 7 test strains,which showed the strongest inhibitory activity against Pseudomonas lachrymans and the weakest on Staphylococcus haemolyticus,and the inhibition zone diameters were (20.0 + 2.0) and (8.7 ± 0.6)mm,respectively.The antibacterial activities of the volatile oil against Ralstoniasolanacearum and Pseudomonas lachrymans were better than that of streptomycin sulfate.The ethyl acetate extracts from Houttuynia cordata showed no antibacterial activity on test bacteria.[Conclusion] Houttuynia cordata was rich in volatile components,and the antibacterial active substances were mainly distributed in the volatile oil.The volatile oil showed better inhibitory activities against Ralstoniasolanacearum and Pseudomonas lachrymans.The inhibitory activity of volatile oil on gramnegative bacteria was stronger than gram-positive bacteria.
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