Study on Stem Disinfection, Bud Induction and Proliferation of Hylocereus undatus in Vitro Culture
[Objective] Hylocereus undatus stem disinfection,bud induction and proliferation were explored during tissue culture to provide technical support for mass seedling production.[Method] Taken stem of Hylocereus undatus cultivar Guihonglong No.1 as the explants,the effects of different factors on stem disinfection,bud inducement and proliferation were studied.[Result] Stem size was the main factor affecting the pollution rate of stem disinfection,followed by the effect of sterilization time and stem mature degrees.The sterilization time had the greatest influence on the bud induction,followed by the 6-BA concentration.Both the stem mature degrees and stem size had little effect on bud induction.6-BA 4.0 mg/L had the best induction effect.The higher concentration of 6-BA showed the higher multiplication ratio of buds,but when 6-BA concentration reached 4.0 mg/L,the buds appeared vitrification.The effect of IAA was better than that of NAA,and the effect of bud proliferation was better when IAA was 0.1 mg/L.PP333 was better than AC in inhibiting vitrification and promoted subculture multiplication of Hylocereus undatus.[Conclusion] The optimal treatment for stem disinfection and bud induction was suggested as:sliced the health stems of about 1 months into pieces with single bud,and disinfected them with 0.1％ HgCl2 solution,and inoculated the explants in medium of MS + 6-BA 4.0 mg/L.The optimal medium for bud proliferation was MS +6-BA 3.0 mg/L + IAA 0.1 mg/L + PP333 1.0 mg/L.
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