Pathogen Identification, Pathogenicity and Biological Characters of Anthracnose on Cymbidium sinense in Yunnan
[Objective] The pathogen of anthracnose on Cymbidium sinense from Pinghian County,Yunnan and its taxonomic status,pathogenic spectrum,biological characteristics were detected,which would lay foundation to protect wild C.sinense.[Method] Tissue isolation,wounding inoculation,morphological and molecular characteristics were used to detect taxonomic status and pathogenic spectrum the pathogen of anthracnose on C.sinense.In additional,the effects of carbon sources,nitrogen sources,temperature,pH value,light on mycelial growth and conidial production were done by growth rate method and hemocytometer measurement.[Result]Two isolates of anthracnose on Cymbidium sinense from Pingbian,Yunnan province were identified as Colletotrichum gloeosporioides by morphological characteristics,symptoms of re-inoculated on C.sinense and phylogenetic analysis based on ITS rDNA regions.In addition,the pathogen could also infect the leaves of Cypripedium corrugatum,C.ensifolium and C.wenshanense.The most suitable carbon and nitrogen source for mycelia growth were mannitol and glycine respectively,the most suitable temperature was 30 ℃,the lethal temperature was 55 ℃,the most suitable pH value was 8,and the most suitable light was dark.The most suitable carbon and nitrogen source for reproduction spore were maltose and peptone respectively,the most suitable temperature was 35 ℃,the lethal temperature was 60 ℃,the most suitable pH value was 7,light didn't affect the reproduction of spore.[Conclusion]The pathogen of anthracnose on Cymbidium sinense from Pingbian county,Yunnan was Co.gloeosporioides,which also infected Cypripediurn corrugatum Franch.,C.ensifolium (L.) Sw.,C.wenshanense Y.S.Wu et F.Y.Liu,was well-adapted envirement.Thus,Cyp.corrugatum,C.ensifolium,C.wenshanense should be forbidden to cultivate in Daweishan Mountain in Pingbian County.
Anthracnose on Cymbidium sinenseColletotrichum gloeosporioidesBiological characteristics
NETL
NSTL
万方数据
维普
