Construction of Molecular Fingerprint Pattern and Genetic Clustering Analysis in Sorghum Forage
[Objective] This study aimed to establish the rapid and effective analysis method for identification of Sorghum forage varieties.[Method] The digital fingerprint,identification and clustering of 12 forage varieties of Sorghum bicolor,Sorghum dochna,Sorghum Sudanense and Sorghum bicolor × S.sudanense are respectively analyzed by the ISSR molecular marker technique based on the mixed DNA pool [Result] 13 amplification products with high polymorphism and good repeatability are screened from 110 ISSR primers by ISSR.The genetic similarity coefficient of 12 forage varieties is 0.6867-0.9036.When Gs is 0.786,12 forage varieties can be divided into four groups.There are six S.bicolor × S.sudanense varieties and two S.bicolor varieties in Group Ⅰ,two S.dochna varieties in Group Ⅱ,one S.sudanense variety in Group Ⅲ and one S.sudanense variety in Group Ⅳ.Three core primers (S37,S35 and S50) are selected to differentiate ISSR digital fingerprint of 12 Sorghum forage varieties.[Conclusion] The ISSR molecular marker technique can effectively identify the purity and true or false of Sorghum forage varieties and reveal genetic difference among Sorghum forage varieties.
NETL
NSTL
万方数据
维普
