Chloroplast genome characteristics of Guiding Niaowang tea
[Objective]The complete chloroplast genome sequence of Guiding Niaowang tea was analyzed to provide a basis for the study of its phylogenetic relationship.[Method]The chloroplast genome of Guiding Niaowang tea of three copies was sequenced,assembled and annotated by the high-throughput sequencing technology,and its phylogenetic analysis with the 23 species of camellia such as Camellia sinensis cultivar'Xinyang 10',C.sinensis cultivar'Youhuaxiang',C.sinensis cultivar'Bantianyao'and so on from NCBI was carried out.[Result]The whole length of the chloroplast genome of Guiding Niaowang tea was 157 071 bp,and the contents of GC and AT were 37.29％and 62.71％,respectively.A total of 138 genes related to photosynthesis and self-reproduction,including 89 protein-coding genes,such as atpA,atpB and atpE,8 rRNA genes,such as rrn5,rrn4.5 and rrn23 and 41 tRNA genes,such as trnA-FME,trnC-GCA and trnD-GUC were annota-ted.The analysis of codon usage showed that the highest and lowest number of codons were leucine and 1-cysteine,respectively,and the pref-erence of chloroplast coding genes of this species ended with A/U(T).SSR analysis showed that there were abundant SSR markers in the tea,which could provide a foundation for the development of SSR primers and phylogeny.The contraction and expansion of the IR region boundaries showed that four boundary areas of IR,including JLB,JSB,JSA and JLA,were identical with C.sinensis cultivar'Xinyang 10',which both had not contraction and expansion status.However,Guiding Niaowang tea had different degrees of expansion and contraction in comparison with the other 7 varieties(C.sinensis cultivar'Youhuaxiang',C.sinensis cultivar'Qiancha 1',C.sinensis cultivar'Bantian-yao',C.sinensis cultivar'Baijiguan',C.sinensis cultivar'Baiye 1',C.sinensis var.dehungensis,C.sinensis cultivar'Longjing 43').Phylo-genetic analysis showed that the closest genetic relationship with the Guiding Niaowang tea was C.sinensis cultivar'Xinyang 10'.[Conclu-sion]The study elucidated the chloroplast gene of Guiding Niaowang and its evolutionary relationship with other tea plants,and laid a founda-tion for the study of genetic diversity,population history,phylogeny and genetic relationship of this species.
NETL
NSTL
万方数据
维普
