Composition and diversity of fungi community in rhizosphere soil of Gymnadenia conopsea in Inner Mongolia
[Objective]The study aimed to analyze the diversity of fungi community in the rhizosphere soil of Gymnadenia conopsea and its correlation with soil environmental factors,so as to provide reference for exploring the work of resource protection and sustainable utilization such as artificial cultivation and ecological planting of G.conopsea.[Method]High-flux sequencing and comparative analysis were carried out on the diversity of rhizosphere soil fungal communities of G.conopsea in Aruhorqin Banner,Dongwuzhumuqin Banner and Ongniud Banner of Inner Mongolia by using Illumina Miseq high-throughput sequencing technology,and the physical and chemical properties and correlation of plant rhizosphere soil were analyzed.[Result]The rhizosphere soil of G.conopsea was acidic,rich in organic matter and available potassi-um,but low in nitrogen and phosphorus,and the soil physical and chemical properties in different distribution areas were significantly differ-ent.A total of 5125 OTUs were obtained from soil samples from three distribution areas,belonging to 11 phyla,31 classes,65 orders,161 families and 158 genera.At the phylum level,the dominant phyla of fungi in the rhizosphere soil of the three distribution areas were Ascomy-cota and Basidiomycota.At the genus level,Trichoglossum,Hygrocybe and Mortierella were the dominant groups of G.conopsea rhizosphere soil fungi.Alpha diversity index showed that the general trend of diversity and richness of rhizosphere soil fungal community in G.conopsea in the three distribution areas was A1＞D3＞W2;Beta diversity index showed that there were some differences in the composition of true communities in rhizosphere soil of G.conopsea in the three distribution areas.The contents of total potassium,total phosphorus,total nitro-gen and water in the soil may be the key factors affecting the composition of the fungal community in the rhizosphere soil of G.conopsea dis-tributed in Inner Mongolia.Among them,Hygrocybe and Clavulinosis had significant positive correlation with the key factors of soil environ-ment;However,there was a significantly negative correlation between the key factors of soil environment and the pathogenic species of Hy-phomycea and Fusarium.[Conclusion]Through the results of the study,we have a preliminary understanding of the growth soil conditions and characteristics of the rhizosphere soil fungal community of G.conopsea distributed in Inner Mongolia.Among the three different distribu-tion areas,the diversity and richness of the rhizosphere soil fungal community of G.conopsea in Aruhorqin Banner are the highest.Soil phys-ical and chemical properties have a great impact on the diversity of fungi in the rhizosphere soil of G.conopsea,and other influencing factors need to be further studied.
万方数据
维普
