Effect of planting years on soil microbial community of Polygonum capitatum
[Objective]The study aimed to investigate the change trend of soil microbial(bacteria and fungi)community composition and species diversity of Polygonum capitatum in different planting years,and to explore the obstacles mechanism of continuous cropping from the perspective of soil microorganisms,thus provide theoretical basis for the sustainable development of P.capitatum industry in Guizhou prov-ince.[Method]Illumina sequencing technology was used to study the effects of planting years on soil microbial community of P.capitatum,using P.capitatum and corn crop rotation plots as CK,the soil microorganisms of P.capitatum as continuously planted for 2 years(T2),3 years(T3)and 4 years(T4)were sequenced and analyzed.[Result]With the increase of continuous cropping years,the abundance of bac-terial community increased from 1620.30 to 1787.22,and the species diversity gradually stabilized.While the fungal community abundance decreased from 635.00 to 612.89,and species diversity changed little.At the level of bacterial community,Proteobacteria and Acidobacte-ria were the dominant bacterial groups,and their relative abundance accounted for more than 64％.With the increase of continuous cropping years,the relative abundance of Proteobacteria decreased significantly(from 40.80％to 34.80％),and Acidobacteria increased significant-ly(from 24.20％to 29.10％).The relative abundance of Proteobacteria(40.80％,40.20％,37.60％and 34.80％)and Planctomyce-tes(1.50％,1.50％,2.60％and 2.50％)were significantly different in different continuous cropping years.At the genus level,the pro-portion of uncultured_bacterium_c_Subgroup_6 was the largest,ranging from 10.66％to 20.50％.At phylum level,Ascomycota and Basid-iomycetes were the dominant fungi groups,and their relative abundance accounted for more than 61％,which decreased year by year com-pared with CK.Mortierellomycota and Chytridomycetes almost disappeared after the fourth year of continuous cropping(T4).At the genus level,Mortierella accounted for the largest proportion,ranging from 2.66％to 14.30％,and Polysporyces appeared in the third year(T3)of continuous cropping.[Conclusion]Continuous cropping increased the difference of microbial communities in planting soil of P.capita-tum.With the increase of continuous planting years,the relative abundance of Proteobacteria and the genus Mortierella in the phylum Mor-tierellomycota gradually decreased,Acidobacteria and the genus Fusarium in the phylum Ascomycota gradually increased,which may be one of the reasons for hindering the continuous cropping of P.capitatum.
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