Effect of dark septal endophytes on growth,photosynthetic capacity,chlorophyll fluorescence and ion absorption of Pinus bungeana seedlings under salt stress
[Objective]The study aimed to investigate the effects of inoculating dark septal endophytes(DSE)on the growth,photosynthetic capacity,chlorophyll fluorescence and ion absorption of Pinus bungeana subjected salt stress.[Method]The trial included NaCl stress(0％,0.1％,0.2％,0.3％)and inoculation treatment:Paraphoma radicina(Pr),Phialophora mustea(Pm),Alternaria tenuissima(At),Acroca-lymma vagum(Av)and unvaccinated control(CK).The infection rate,plant biomass,photosynthetic capacity,chlorophyll fluorescence,K+and Na+content were measured after 30 days of NaCl treatment.[Result]The infection rate of Pr,Pm,At,and Av was highest at NaCl stress level of 0.3％,0.2％,0.1％,0.2％,respectively.Compared to seedlings subjected to 0％NaCl stress,those exposed to 0.3％NaCl stress exhibited significant increases in shoot,root and total biomass.Inoculation treatments led to elevated total chlorophyll content,en-hanced photosynthetic parameters,increased TRo/RC,ETo/RC ratios,as well as decreased ABS/RC,DIo/RC in chlorophyll fluorescence.Notably,under 0.3％NaCl stress,Pm,At and Av treatments significantly reduced shoot Na+content in P.bungeana,by 34.0％,26.6％and 26.3％,respectively.Furthermore,Pm and Av treatments considerably decreased root Na+content,by 24.4％and 26.6％respective-ly.At 0.2％NaCl stress,Pr,Pm,At,Av significantly increased K+content by 42.9％,58.99％,56.96％and 55.92％respectively,consequently elevating the K+/Na+ratio.[Conclusion]Moderate NaCl stress was found to enhance the infection rate of DSE.The DSE in-oculation was observed to mitigate the impact of NaCl stress on the photosynthesis,regulate K+and Na+ion content,and thereby alleviate NaCl-induced damage while sustaining growth.
Dark septal endophytePinus bungeanaNaCl stressGrowthPhotosynthetic fluorescenceIon absorption
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