Effects of grafting on soil microbial community compositions in rhizospheres of tomatoes
[Objective]The aim of the study was to explore the effects of grafting on the rhizosphere microbial community of tomato plants,in order to reveal the mechanism of enhancing resistance of tomato plants by grafting,and to provide reference for constructing a system to solve obstacle of tomato continuous cropping.[Method]Based on Misseq high-throughput sequencing technology,Xinzhen 1 and Baozhen 1 culti-vars were used as rootstocks,and Taotailang cultivar was used as scion.Xinzhen 1 plant(abbreviated as Xinzhen);Xinzhen 1 × Taotailang grafted plant(abbreviated as Taotailang/Xinzhen);Baozhen 1 plant(abbreviated as Baozhen 1);Baozhen 1 × Taotailang grafted plant(abbreviated as Taotailang/Baozhen 1);Taotailang plant(abbreviated as Taotailang).[Result]Compared with Xinzhen,Baozhen and Tao-tailang plants,the number of operational taxonomic unit(OTU)of the bacterial and fungal communities in Taotailang/Xinzhen and Tao-tailang/Baozhen changed greatly.Compared with rootstock and scion plants,the rhizosphere soil of tomato grafted plants had more abundant microbial OTU and specific OTU.There were no significant differences in the diversity and richness of bacteria and fungi in rhizosphere soil between tomato plants and grafted plants(P＞0.05).At the phylum classification level,the abundance of Actinobacteria and Firmicutes in rhizosphere soil of Taotailang/Xinzhen plants was higher than that of Xinzhen and Taotailang plants.The bacterial abundance of Chloroflexi,Acidobacteria and Gemmatumonadetes in Taotailang/Baozhen plants were higher than Baozhen and Taotailang plants.At the genus level,compared with the Xinzhen and Taotailang plants,the abundance of Sphingomonas,Bacillus,Streptomyces,unclassified_f__Micrococcaceae and norank_f__norank_o__Gaiellales in rhizosphere soil of Taotailang/Xinzhen plants increased.Compared with Taotailang plants,endemic dominant bacteria(Marmoricola)were found in rhizosphere soil of Taotailang/Xinzhen plants.Compared with the Banzhen and Taotailang plants,in the rhizosphere soil of Taotailang/Baozhen plants,the abundance of norank_f__norank_o__norank_c__Subgroup_6,Nocardio-ides,norank_f__norank_o__norank_c__Gitt-GS-136,norank__f__norank_o__norank_c__KD4-96,norank_f__JG30-KF-CM45 in-creased,while the endemic dominant bacteria norank_f__norank_o__norank_c__JG30-KF-CM66 were found.At the level of fungi classifi-cation,compared with tomato plants,the abundance of Chytridiomycota fungi in the rhizosphere soil of grafted plants increased,while the a-bundance of Ascomycota fungi decreased.In addition,the abundance proportion of unclassified_k__Fungi in the rhizosphere soil of Tao-tailang/Xinzhen grafted plants increased,while the abundance proportion of unclassified_k__Fungi in the rhizosphere soil of Taotailang/Baozhen grafted plants decreased.At the genus level,compared with the corresponding mature plants,the rhizosphere soil of Taotailang/Xinzhen plants showed an increased abundance proportion of Rhizochytis,unclassified_k__Fungi and Cladospora fungi.Trichoderma,Asper-gillus,unclassified_o__Eurotiales and unclassified_o__Sordariales were missing in rhizosphere soil of Taotailang/Xinzhen plants,but Fusar-ium was added in plants rhizosphere soil compared with Xinzhen plants.In the rhizosphere soil of Taotailang/Baozhen plants,the abundance of Rhizohlytis and Penicillium increased,while the abundance of Cladosporium,Trichoderma,Aspergillus,Fusarium,unclassified_o__Euro-tiales and Arachniotus were missing.[Conclusions]Compared with plants,grafting not only changed the abundance proportion of rhizosphere soil microbial community,but also changed the composition of rhizosphere soil microbial community.The improvement of rhizosphere soil mi-crobial community of tomato grafted plants is one of the important reasons for the increased resistance of tomato grafted plants.
TomatoGraftingRhizosphereMicrobial community compositions
NETL
NSTL
万方数据
