Bioinformatics and tissue expression analysis of DPP6 and PRKN in Holstein
[Objective]The purpose of the study was to explore the biological properties and tissue expression characteristics of Dipeptidyl Peptidase-like 6(DPP6)and E3 ubiquitin ligase gene(PRKN)genes that were screened by GW AS in the previous stage and related to milk production traits of Holstein.The study provided a basis for further verification of the regulatory mechanisms of DPP6 and PRKN genes on milk-production-related traits in Holstein.[Method]The physicochemical properties and protein structure of DPP6 and PRKN were analyzed by ProtParam,Phyre2,NetPhos 3.1 and other software,and Real-Time quantitative PCR was used.The expression patterns of DPP6 and PRKN in the heart,spleen,liver,lung,kidney,mammary gland,uterus,ovary,rumen and luteum of Holstein were detected by RT-qPCR;The relationship between DPP6 and PRKN genes and udder inflammation in Holstein dairy cows was examined by means of lipopo-lysaccharide(LPS)induced mammary epithelial cells(bMECs).The expression patterns of DPP6 and PRKN genes in inflammatory re-sponse were preliminarily detected.[Result]DPP6 protein encoded 863 amino acids and was closely related to goat.PRKN protein encoded 488 amino acids,among which glycine content was the highest,its sequence was the most similar to that of red deer,and the conserved type was strong.The study speculated that the DPP6 and PRKN proteins of two species may have similar biological functions,and both proteins were unstable hydrophilic proteins.DPP6 and PRKN genes were widely expressed in tissues.The expression of DPP6 in breast,lung,spleen and kidney tissues was significantly higher than that in other tissues,while the expression of PRKN was significantly higher in breast,kidney and rumen tissues.In addition,both DPP6 and PRKN genes were significantly expressed in LPS-induced bMECs compared with the blank control group(DPP6 gene was significantly down-regulated,and PRKN gene was highly upregulated).[Conclusion]DPP6 and PRKN genes may play a potential role in the regulation of udder inflammation in Holstein dairy cows,and provide a theoretical basis for the subsequent study of the regulatory mechanisms of DPP6 and PRKN genes in bMECs inflammation in dairy cows.
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