Comprehensive evaluation on drought resistance of thirty Saccharum spontaneum L.accessions in seedling stage
[Objective]In order to provide reference for parent selection of drought resistance breeding in sugarcane,physiological and bio-chemical indices were used to evaluate the drought resistance of different genotypes of Saccharum spontaneum in seedling stage.[Method]Thirty S.spontaneum accessions were used as the materials under the normal water and drought stress treatment,and six physiological and biochemical indices were determined at the seedling stage.Comprehensive evaluation of drought resistance was implemented by the methods of principal component analysis,fuzzy membership function analysis and cluster analysis.[Result]Compared with the normal water suppy,the activities of CAT,SOD,POD and the contents of MDA,SS and Pro increased under drought stress,but the changes of each index were diferent due to diftrent materials.The results of correlation analysis showed that under drought stress,CAT activity of leaves was significantly positively correlated with Pro content(P＜0.05);SOD activity of leaves was extremely positively correlated with Pro content(P＜0.01);There was a significant negative correlation between POD activity and SOD activity;There was also a significant negative correlation between catalase activity and MDA content,while there was no significant correlation between other indicators(P＞0.05).The principal component analysis showed that the activities of SOD,POD,CAT and the contents of Pro,SS,MDA could be used as important indicators for drought re-sistance evaluation.Fuzzy membership function analysis and cluster analysis showed that the 30 accessions could be divided into 4 catego-ries,including 1 accession as the high drought-resistant type(90-53),2 accessions as the medium high drought-resistant type,14 accessions as the drought-resistant type and 13 accessions as the drought-sensitive type.[Conclusion]The activities of CAT,SOD,POD and the con-tents of MDA,SS and Pro could be used as the drought resistance evaluation index of S.spontaneum at the seedling stage.Among the 30 ac-cessions tested,the 90-53 had the strongest drought resistance,followed by 2015-22 and 88-301,2015-104 had the weakest drought resist-ance.
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