Analysis of fungal changes in Gastrodia elata planting soil based on high throughput sequencing
[Objective]The study aimed to reveal the changes of soil fungal community structure of Gastrodia elata,the changes of soil fungi community before and after G.elata planting,and the changes of soil fungi after G.elata planting,so as to provide the theoretical basis for studying the rhizosphere microecology of G.elata,and the technical support for solving the obstacle of continuous cropping of G.elata in the future.[Method]Genomic DNA extraction,the PCR amplification,product detection,purification and quantification and the species compo-sition diversity and difference analyses were performed on three groups of the soil samples(the original soil charlle00 1,a cropping of soil charlle002,and restoration soil charlle006)based on the high-throughput sequencing method.[Result]The fungi in the three soil samples mainly belonged to phyla Ascomycota,Basidiomycota and Mortierellomycota,and Ascomycota had the largest fungal abundance.Specific fungus OTUs of 3 groups of samples showed:a croping soil＞bamboo soil＞original soil.The main dominant fungi were Clitocybe sp.and Solicoccozyma sp.in charlle00 1,and Spirosphaera sp.and Clavulinopsis sp.were in charlle002,and Trichoderma sp.was in charlle006.Clavulinopsis and Rosellinia had maximum abundance in charlle002 and minimal abundance in charlle00 1 and charlle006.The relative abun-dance of Mortierella was significantly different between charlle00 1 and charlle002,and Penicillium was significantly different between char-lle001 and charlle006,and Penicillium was significantly different between charlle002 and charlle006.There was a significant increase of Tri-choderma in bamboo soil.Some fungi of Clavulinopsis and Rosellinia caused gastrodia diseases,while Trichoderma was used for biological control of pathogens in soil.[Conclusion]The fungal community structure of different soils planted with G.elata is different,and planting bamboo has a restorative effect,which pushes the process of the specific repaired mechanism of G.elata for further research.
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