栀子WRKY基因家族鉴定及其响应盐胁迫的表达模式
Identification of WRKY gene family in Gardenia jasminoides and its expression patterns in response to salt stress
陈啸 1陈淑颖 1朱渊铭 1倪林 2邹双全1
作者信息
- 1. 福建农林大学林学院,福州 350002;自然生物资源保育利用福建省高校工程研究中心,福州 350002
- 2. 自然生物资源保育利用福建省高校工程研究中心,福州 350002;福建农林大学植物保护学院,福州 350002
- 折叠
摘要
[目的]为进一步研究WRKY基因家族在调控栀子盐胁迫中的功能作用、培育栀子抗盐新品种.[方法]利用生物信息学方法对栀子WRKY基因家族成员(GjWRKY)进行全基因组鉴定和相关分析.[结果]共鉴定出47个WRKY基因,非均匀地分布在10条染色体上,通过系统发育树将其分为3大组:Group Ⅰ、Ⅱ和Ⅲ.GjWRKY基因以串联重复为主要扩增方式,且多数基因成员含有3个外显子.GjWRKY基因家族启动子2000 bp区域含有大量激素类和胁迫类响应顺式作用元件.46个GjWRKY基因在中度(MS)和重度盐胁迫(SS)下具有不同程度的高表达,部分GjWRKY基因产生特异性表达,17个GjWRKY基因表现为上调表达,推测这部分GjWRKY基因可能起正调控作用.[结论]GjWRKY基因在栀子抗盐胁迫中发挥了重要作用.
Abstract
[Objective]The study aimed to further investigate the functional role of the WRKY gene family in regulating salt stress in Gardenia jasminoides and cultivate new salt resistant varieties.[Method]The WRKY gene family members of G.jasminoides(GjWRKY)were identified and analyzed by bioinformatics.[Result]The study identified a total of 47 WRKY genes,which were unevenly distributed on 10 chromo-somes.They were divided into Group Ⅰ,Ⅱ and Ⅲ groups by phylogenetic tree.The GjWRKY gene family was mainly amplified by tandem re-peat,and most members of the gene family contained three exons.The 2000 bp region of the GjWRKY gene family promoter contained a large number of hormone and stress responsive cis-acting elements.46 GjWRKY genes had different degrees of high expression under moderate(MS)and severe salt stress(SS),and some GjWRKY genes had specific expression,17 GjWRKY genes expressions were up-regulated,sug-gesting that these GjWRKY genes may play a positive role in regulation.[Conclusion]The above indicates that the GjWRKY genes plays an important role in the salt stress resistance of G.jasminoides.
关键词
栀子/WRKY基因/盐胁迫/生物信息学/系统进化分析Key words
Gardenia jasminoides/WRKY gene/Salt stress/Bioinformatics/System evolutionary analysis引用本文复制引用
基金项目
福建省林业科技推广项目(2022TG09)
福建省科技厅高等学校产学合作项目(2022N5009)
出版年
2024
国家科技期刊平台
NSTL
万方数据