Effect of topping on bacterial community structure and function in rhizosphere soil of tobacco
[Objective]The study aims to further elucidate the effects of plant topping measures on rhizosphere microbial community structure and potential functions,and to provide necessary theoretical basis for the optimization of topping technology.[Method]The impact of tobacco plant topping on rhizosphere bacterial community structure and potential function was investigated using 16S rRNA high-throughput sequen-cing technology.[Result]Topping induced changes in the content of nitrogen,phosphorus,and potassium in leaves of flue-cured tobacco.Compared to the control,topping significantly increased the potassium content in tobacco leaves,while decreasing the nitrogen and phosphor-us contents.The contents of organic matter,total nitrogen,hydrolyzed nitrogen and available potassium in soil increased significantly after topping,while the soil pH value decreased significantly.The contents of total phosphorus,total potassium and available phosphorus in soil did not change significantly before and after topping.Topping increased the relative abundance of Gemmatimonadetes and Acidobacteria,while decreasing the relative abundance of Proteobacteria,Actinobacteria,Sphingomonas,Gemmatimonas,and Lysobacter.α-diversity and NMDS analysis revealed that topping altered the uniformity and richness of the soil bacterial community,changing its structure significantly.Topping notably increased the Sob index and Chao1 index,causing significant separation of the community structure.Redundancy analysis(RDA)and variance decomposition analysis(VPA)indicated that soil nutrient change was a crucial factor in rhizosphere microbial commu-nity alteration.Soil physical and chemical properties were closely correlated with microbial community changes,with soil total nitrogen and hydrolyzed nitrogen exerting the greatest influence on microbial communities.LEfSe analysis demonstrated that topping resulted in a signifi-cant increase in bacterial diversity.Functional prediction indicated that topping led to a decrease in the relative abundance of soil chemohet-erotrophic and oxidoheterotrophic bacteria.[Conclusion]Topping induces significant alterations in the structure and potential functions of the rhizosphere microbial community.The changes in rhizosphere soil nutrients caused by topping may constitute a key factor influencing the var-iations observed in the rhizosphere bacterial community.
ToppingPhysical and chemical properties of soilSoil bacteria
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