Composition characteristics of antibiotic resistance genes in sediments and water in Dongzhaigang mangrove
[Objective]The study explored the distribution of antibiotic resistance genes(ARGs)in mangroves and the association between microorganisms and ARGs in order to understand the current situation of mangrove resistance pollution and provide theoretical support for risk assessment of ARGs in the environment.[Method]Metagenomics technique was used to explore the composition,diversity and relationship with microorganisms of antibiotic resistance genes in mangrove sediments and water in Dongzhaigang,Hainan province.[Result]The rifampi-cin resistance gene rpoB mutants,daptomycin resistance gene rpoC,fluoroquinolone resistance gene mfd,aminocoumarin resistance gene a-laS,acetoxy resistance gene EF-Tu mutants,macrolide resistance gene macB and isoniazid resistance gene katG were dominant genes in this area.The composition types of antibiotic resistance gene in sediment and water were similar,but the diversities of antibiotic resistance gene in water were higher than those in sediment.However,NMDS analysis showed that the composition and distribution of ARGs in sediments and water bodies were obviously different.The correlation analysis between ARGs and microbial groups showed that α-Proteobacteria,γ-Pro-teobacteria,β-Proteobacteria and Flavobacteriia were the main microbial groups strongly associated with ARGs,accounting for 70％.Among them,the resistance genes in water were mainly distributed in α-Proteobacteria,β-Proteobacteria and Flavobacteriia,while the resistance genes in sediments were mainly distributed in y-Proteobacteria and δ-Proteobacteria.[Conclusion]Mangrove sediments and water bodies have different composition characteristics of ARGs,and they both have different risk characteristics.As a result,when carrying out ecological mo-nitoring and management of drug-resistant microorganisms and ARGs in mangroves,attention should be paid not only to monitoring the com-positional characteristics of microorganisms,but also to the pattern of change of ARGs in the water body,in order to predict the risk of drug-resistance of microorganisms in sediment in this area of mangroves in Dongzhaigang,Hainan.
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