[Objective]The present study aims to screen candidate genes related to muscle growth and development of Tan sheep with different genders by RNA-Seq technology,and initially reveal the molecular mechanism of the formation of differences in longisone dorsi muscle of Tan sheep,which can provide theoretical basis for the study of muscle growth and development.[Method]8-month-old Tan sheep under the same feeding conditions were selected as the research objects,and transcriptome sequencing was used to analyze and compare the quality of the longest back muscle of Tan sheep.mRNA transcriptome sequencing and analysis were performed using Illumina HiSeqTM4000 platform,and relevant regulatory genes with different effects on muscle growth and development were discovered.The differential genes were verified by RT-qPCR.[Result]A total of 37 differentially expressed genes were screened by RNA-Seq technology,including 10 up-regulated genes and 27 down-regulated genes.The GO function annotation analysis of differential genes showed that they were significantly enriched in 41 GO i-tems(P＜0.05).Meanwhile,KEGG enrichment analysis showed that DEGs was enriched in 28 pathways,including FoxO,PI3K-Akt,regulation of stem cell pluripotency,purine metabolism,actin cytoskeleton regulation,Notch signaling,protein digestion and absorption,calcium signaling and other pathways.Furthermore,six genes including KSR2,ENAH,COL9A1,IFIT1,B3GAT2 and TACR1 were associ-ated with muscle growth and development.Real-time fluorescence quantitative PCR was performed to verify the expression of 6 differential genes,and the results were consistent with the expression trend of transcriptome sequencing,indicating that the sequencing results were reli-able.[Conclusion]The candidate genes KSR2,ENAH,COL9A1,IFIT1,B3GAT2 and TACR1 obtained in the study can provide more refer-ence materials for muscle growth and development,thus laying a theoretical foundation for subsequent research on muscle growth and devel-opment of high-quality meat sheep in Ningxia.
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