Transcriptome analysis of colored potato in response to UV-B radiation
[Objective]The study aimed to explore the mechanism behind the strong tolerance of colored potatoes to UV-B radiation,and to analyze the molecular mechanism of potatoes'response to UV-B radiation through transcriptome analysis,therefore providing theoretical basis for screening genes related to UV-B radiation resistance in potatoes.[Method]The colored potato'Huaxinyangyu'was treated with UV-B ra-diation throughout the growth period,and tubers at the maturity stage were selected for sequencing analysis via the Illumina HiSeq2500 plat-form.[Result]A total of 232 285 320 effective sequencing reads were obtained,and the percentage of comparison with the reference genome was more than 78.5％,while the percentage of GC content was more than 42.1％.The analysis screened 843 differentially expressed genes(DEGs),including 544 up-regulated genes and 299 down-regulated genes.GO functional analysis showed that DEGs in'Huaxinyangyu'were mainly enriched in metabolic processes,cellular components and catalytic activities.KEGG enrichment analysis revealed that'Huaxin-yangyu'mainly responded to UV-B radiation through the carbohydrate metabolic pathway.The transcription factors involved were mainly ARR-B,AP2-EREBP,MADS,bHLH and NAC,which were related to the response to light signals and environmental stress.[Conclusion]The main pathway involved in the response to UV-B radiation is the carbohydrate metabolism pathway,and the transcription factors ARR-B,AP2-EREBP,MADS,bHLH and NAC all play certain regulatory roles.
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