Accumulation of sanshool in fruits of Zanthoxylum armatum DC.and Z.bungeanum in different mature period
[Objective]The experiment was conducted to study the accumulation and changes of sanshool at different maturity stages,and pro-vide reference for utilization of functional components in Zanthoxylum armatum DC.and Z.bungeanum.[Method]Taking fresh fruit of Zan-thoxylum armatum DC.and Z.bungeanum as test material,sanshool was extracted with methanol,and HPLC-MS technology was used to con-tinuously detect sanshool at different maturity stages.[Result]Sanshool of Z.armatum DC.was 7.7-19.6 mg/g,which showed a trend of upward trend followed by a downward trend;In the early stage of maturity,the content of sanshool increased rapidly,with an increase of 127.92％.The fluctuation during the maturity period reached its peak on June 12th,and in the late stage of maturity,it rebounded by 26.32％.Sanshool of Z.bungeanum was 0.42-17.06 mg/g,showing a trend of continuous accumulation and increase,the fastest in the young fruit stage of sanshool,with an increase of 19.9.It continued to increase rapidly in the early stage of maturity,but the growth rate nar-rowed and reached its peak on July 28th.The main components of sanshool of the Z.armatum DC.and Z.bungeanum were hydroxy-α-san-shool,hydroxy-β-sanshool,hydroxy-γ-sanshool and hydroxy-ε-sanshool,and hydroxy-α-sanshool content was the highest.Hydroxy-ε-sanshool of Z.armatum DC.and Z.bungeanum were highly consistent on dynamic changes.There were significant differences among the other three components;Hydroxy-β-sanshool were the maximum variable in sanshool of Z.armatum DC.and Z.bungeanum.Hydroxy-β-sanshool in-creased significantly and decreased rapidly in early stage of maturity and stage of maturity Z.armatum DC.,in early stage of maturity Z.bun-geanum and was the key factor affecting content changes of Z.armatum DC.sanshool in early stage of maturity and stage of maturity.[Con-clusion]The content of sanshool of Z.armatum DC.and Z.bungeanum and components showed dynamic changes at different maturity stages,has obvious differences;Based on the accumulation and changes of sanshool and components,June 12th is selected as sanshool and hydroxy-α-sanshool,hydroxy-β-sanshool in Z.armatum DC.and the hydroxy-β-sanshool in Z.bungeanum.July 13th is the optimal research and utili-zation period of the hydroxy-α-sanshool of Z.bungeanum and hydroxy-γ-sanshool of Z.armatum DC.;July 28 th is the optimal research and u-tilization period of the sanshool and hydroxy-γ-sanshool and hydroxy-ε-sanshool in Z.bungeanum;Mid August is the optimal research and uti-lization period of hydroxy-ε-sanshool in Z.armatum DC..
Different mature periodsSanshoolAccumulate changesComparative analysis
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